SKU: 2684
$17.58 USD
25g
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Description

The 3,5-Dinitrosalicylic Acid Analytical Grade by Dinâmica, available at LojaLab, is a nitroaromatic compound known by the abbreviations DNS or DNSA and used to prepare a colorimetric reagent for reducing sugars. In alkaline medium under heating, reducing substances convert part of the DNS into a colored amino-nitro product, producing an orange to reddish hue whose absorbance can be related to concentration through a calibration curve. The complete reagent normally contains additional components that control alkalinity, stability and color development.

Characteristics

  • Nitroaromatic reagent also called DNS or DNSA
  • Contains carboxyl, phenolic hydroxyl and two nitro groups
  • Forms a colored product after reduction in alkaline medium under controlled heating
  • Applied to reducing-sugar assays and hydrolytic-enzyme activity measurements
  • CAS 609-99-4, formula C7H4N2O7 and molar mass 228.12 g/mol
  • Colorimetric method depends on formulation, calibration curve, time and temperature.

Applications

  • Preparation of DNS reagent for colorimetric determination of reducing sugars
  • Indirect quantification of glucose, maltose and reducing equivalents using an appropriate curve
  • Assessment of amylase, cellulase, xylanase and other glycosidase activities that release reducing sugars
  • Monitoring hydrolysis of starch, cellulose and other carbohydrates in process studies
  • Research on interferences, optimization and adaptation of the DNS assay to different matrices.

Technical Specifications

  • Product: 3,5-Dinitrosalicylic Acid Analytical Grade
  • Brand: Dinâmica Química
  • Grade: Analytical grade
  • CAS number: 609-99-4
  • Chemical formula: C7H4N2O7
  • Molar mass: 228.12 g/mol
  • Available quantity: 25 g.

Technical Data

Chemical identity3,5-Dinitrosalicylic acid
Common abbreviationsDNS or DNSA
Chemical classHydroxydinitrobenzoic acid
Physical stateYellow to yellow-orange solid
Analytical principleReduction in alkaline medium with color development after heating
MeasurementWavelength and timing defined by formulation and instrument
LimitationNonspecific response; Requires blank, curve and interference control

Frequently Asked Questions

What is 3,5-Dinitrosalicylic Acid Analytical Grade used for?

The main applications of 3,5-Dinitrosalicylic Acid Analytical Grade include preparation of DNS reagent for colorimetric determination of reducing sugars; indirect quantification of glucose, maltose and reducing equivalents using an appropriate curve.

What are the main characteristics of 3,5-Dinitrosalicylic Acid Analytical Grade?

The characteristics listed for 3,5-Dinitrosalicylic Acid Analytical Grade include nitroaromatic reagent also called DNS or DNSA; contains carboxyl, phenolic hydroxyl and two nitro groups.

How should 3,5-Dinitrosalicylic Acid Analytical Grade be selected for a laboratory application?

Selection of 3,5-Dinitrosalicylic Acid Analytical Grade should consider the grade, presentation, method requirements, and technical specifications described on this page.

What quantities of 3,5-Dinitrosalicylic Acid Analytical Grade are available?

3,5-Dinitrosalicylic Acid Analytical Grade is available in 25 g. For other quantities, recurring purchases, or larger requirements, contact the sales team to check availability and request a quotation.

How can companies and institutions purchase 3,5-Dinitrosalicylic Acid Analytical Grade and request a formal quotation?

Companies, laboratories, educational institutions, and research organizations can purchase 3,5-Dinitrosalicylic Acid Analytical Grade directly from LojaLab. For recurring purchases, internal approval processes, research funding, public procurement, or formal quotation requirements, contact the sales team.

Professional Use and Safety Guidance

This product is intended for professional use in scientific research, laboratory analysis, education, and compatible industrial applications.

Each product has its own handling, storage, and disposal requirements. Before use, consult the corresponding SDS and follow the manufacturer's instructions and the safety procedures adopted by the institution.

LojaLab provides guidance on product selection and provenance; defining the use protocol and verifying suitability for the final application are the responsibility of the purchasing professional or institution.