The Coomassie Brilliant Blue G-250 (CI 42655) by Dinâmica, available at LojaLab, is a triarylmethane dye used for protein detection and quantitation. In acidic medium, binding to basic and aromatic protein residues produces a color change and absorbance shift, which is the principle of the Bradford assay and related protein-staining methods.
Characteristics
- G-250 variant of Coomassie Brilliant Blue dye
- Basis of the Bradford colorimetric protein assay
- Produces a spectral change after binding to proteins in acidic medium
- May also be used in colloidal formulations for protein gel staining.
Applications
Coomassie Brilliant Blue G-250 may be used in:
- Preparation of reagents for Bradford assays
- Spectrophotometric determination of total protein near 595 nm
- Construction of calibration curves using protein standards
- Colloidal staining and visualization of proteins in polyacrylamide gels, according to the selected formulation.
Technical Specifications
- Product: Coomassie Brilliant Blue G-250 (CI 42655)
- Brand: Dinâmica Química
- Color index: CI 42655
- CAS number: 6104-58-1
- Molecular formula: C47H48N3NaO7S2
- Molar mass: 854.02 g/mol
- Available quantity: 25 g.
Frequently Asked Questions
What is Coomassie Brilliant Blue G-250 (CI 42655) used for?
The main applications of Coomassie Brilliant Blue G-250 (CI 42655) include preparation of reagents for Bradford assays; spectrophotometric determination of total protein near 595 nm.
What are the main characteristics of Coomassie Brilliant Blue G-250 (CI 42655)?
The characteristics listed for Coomassie Brilliant Blue G-250 (CI 42655) include G-250 variant of Coomassie Brilliant Blue dye; basis of the Bradford colorimetric protein assay.
How should Coomassie Brilliant Blue G-250 (CI 42655) be selected for a laboratory application?
Selection of Coomassie Brilliant Blue G-250 (CI 42655) should consider the grade, presentation, method requirements, and technical specifications described on this page.
What quantities of Coomassie Brilliant Blue G-250 (CI 42655) are available?
Coomassie Brilliant Blue G-250 (CI 42655) is available in 25 g. For other quantities, recurring purchases, or larger requirements, contact the sales team to check availability and request a quotation.
How can companies and institutions purchase Coomassie Brilliant Blue G-250 (CI 42655) and request a formal quotation?
Companies, laboratories, educational institutions, and research organizations can purchase Coomassie Brilliant Blue G-250 (CI 42655) directly from LojaLab. For recurring purchases, internal approval processes, research funding, public procurement, or formal quotation requirements, contact the sales team.
Professional Use and Safety Guidance
This product is intended for professional use in scientific research, laboratory analysis, education, and compatible industrial applications.
Each product has its own handling, storage, and disposal requirements. Before use, consult the corresponding SDS and follow the manufacturer's instructions and the safety procedures adopted by the institution.
LojaLab provides guidance on product selection and provenance; defining the use protocol and verifying suitability for the final application are the responsibility of the purchasing professional or institution.
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