The Ethidium Bromide by Dinâmica, available at LojaLab, is a cationic fluorescent dye widely used for nucleic acid visualization in electrophoresis, particularly in agarose and polyacrylamide gels. Its fluorescence increases when the molecule associates with DNA or RNA, enabling detection of bands after electrophoretic separation.
Characteristics
- Fluorescent dye with affinity for nucleic acids;
- Molecular formula C21H20BrN3 and approximate molar mass of 394.31 g/mol;
- Increased fluorescence associated with interaction with DNA and RNA.
Applications
- Visualization of DNA in agarose gels after electrophoresis;
- Detection of nucleic acids in polyacrylamide gels and compatible systems;
- Monitoring electrophoretic separations in molecular biology using ethidium bromide as the dye.
Technical Specifications
- Product: Ethidium Bromide
- Brand: Dinâmica
- CAS: 1239-45-8
- Molecular formula: C21H20BrN3
- Molar mass: 394.31 g/mol
- Functional class: Fluorescent nucleic-acid dye
- Available quantity, volume, or capacity: 1 g and 5 g
Technical Data
| Principle | Fluorescence increases after association with nucleic acids |
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| Targets | DNA and RNA |
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| Primary technique | Gel electrophoresis |
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| Readout | Fluorescence |
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Frequently Asked Questions
What is Ethidium Bromide used for?
Ethidium Bromide is used as a fluorescent dye to visualize DNA and other nucleic acids after separation in agarose or polyacrylamide gels.
What are the main characteristics of Ethidium Bromide?
Ethidium Bromide has molecular formula C21H20BrN3, an approximate molar mass of 394.31 g/mol, and increased fluorescence when associated with nucleic acids.
Why does the fluorescence of Ethidium Bromide increase in the presence of nucleic acids?
When Ethidium Bromide associates with nucleic-acid structures, its molecular environment changes and fluorescent emission becomes stronger, a principle used to visualize DNA and RNA bands in electrophoretic systems.
What quantities of Ethidium Bromide are available?
Ethidium Bromide is available in 1 g and 5 g presentations. For other quantities, recurring purchases, or larger requirements, contact the sales team to check availability and request a quotation.
How can companies and institutions purchase Ethidium Bromide and request a formal quotation?
Companies, laboratories, educational institutions, and research organizations can purchase Ethidium Bromide directly from LojaLab. For recurring purchases, internal approval processes, research funding, public procurement, or formal quotation requirements, contact the sales team.
Professional Use and Safety Guidance
This product is intended for professional use in scientific research, laboratory analysis, education, and compatible industrial applications.
Each product has its own handling, storage, and disposal requirements. Before use, consult the corresponding SDS and follow the manufacturer's instructions and the safety procedures adopted by the institution.
LojaLab provides guidance on product selection and provenance; defining the use protocol and verifying suitability for the final application are the responsibility of the purchasing professional or institution.
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